Original scientific paper

https://doi.org/10.2478/acph-2023-0016

Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells

YUAN CHEN ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
LIQI YIN ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China
MINGXUAN HAO ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China
WENKAI XU ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
JIXIAN GAO ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
YUXIN SUN ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
QIAO WANG ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
SHI CHEN ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
YOUFENG LIANG ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China
RUI GUO ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China
JINKU ZHANG ; Department of Pathology, First Central Hospital of Baoding City, Baoding 071000, Hebei, China; Key Laboratory of Molecular Pathology and Early Diagnosis of Tumor in Hebei Province, Baoding 071000, Hebei, China
JINMEI LI ; Department of Pathology, First Central Hospital of Baoding City, Baoding 071000, Hebei, China; Key Laboratory of Molecular Pathology and Early Diagnosis of Tumor in Hebei Province, Baoding 071000, Hebei, China
QIONGLI ZHAI ; Department of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
RUNFEN CHENG ; Department of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China
JIANSONG WANG ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
HAIFENG WANG ; Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China
ZHAO YANG ; College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, China; College of Life Science and Technology, Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar 843300, Xinjiang, China

Abstract

Bladder cancer (BC) is the tenth most commonly diagnosed cancer. High recurrence, chemoresistance, and low response rate hinder the effective treatment of BC. Hence, a novel therapeutic strategy in the clinical management of BC is urgently needed. Medicarpin (MED), an isoflavone from Dalbergia odorifera, can promote bone mass gain and kill tumor cells, but its anti-BC effect remains obscure. This study revealed that MED effectively inhibited the proliferation and arrested the cell cycle at the G1 phase of BC cell lines T24 and EJ-1 in vitro. In addition, MED could significantly suppress the tumor growth of BC cells in vivo. Mechanically, MED induced cell apoptosis by upregulating pro-apoptotic proteins BAK1, Bcl2-L-11, and caspase-3. Our data suggest that MED suppresses BC cell growth in vitro and in vivo via regulating mitochondria-mediated intrinsic apoptotic pathways, which can serve as a promising candidate for BC therapy.

Keywords

bladder cancer; medicarpin; cell cycle arrest; apoptosis, therapy

Hrčak ID:

286664

URI

https://hrcak.srce.hr/286664

Publication date:

30.6.2023.

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