Short communication, Note
Enhanced Enzymatic Production of Cephalexin at High Substrate Concentration with in situ Product Removal by Complexation
Dengchao Li
; State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China, University of Science and Technology, CN-200237 Shanghai, PR China
Yewang Zhang
; State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China, University of Science and Technology, CN-200237 Shanghai, PR China
Shiwei Cheng
; State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China, University of Science and Technology, CN-200237 Shanghai, PR China
Qiong Gao
; State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China, University of Science and Technology, CN-200237 Shanghai, PR China
Dongzhi Wei
; State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China, University of Science and Technology, CN-200237 Shanghai, PR China
Abstract
Cephalexin (CEX) was synthesized with 7-amino-3-deacetoxycephalosporanic acid (7-ADCA) and D(–)-phenylglycine methyl ester (PGME) using immobilized penicillin G acylase from Escherichia coli. It was found that substrate concentration and in situ product could remarkably influence the ratio of synthesis to hydrolysis (S/H) and the efficiency of CEX synthesis. The optimal ratio of enzyme to substrate was 65 IU/mM 7-ADCA. High substrate concentration improved the 7-ADCA conversion from 61 to 81 % in the process without in situ product removal (ISPR), while in the synthetic process with ISPR, high substrate concentration increased the 7-ADCA conversion from 88 to 98 %. CEX was easily separated from CEX/β-naphthol complex and its purity and overall yield were 99 and 70 %, respectively.
Keywords
cephalexin; complexation; enzymatic synthesis; in situ product removal; penicillin G acylase
Hrčak ID:
30425
URI
Publication date:
17.11.2008.
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