Review article

N-Glycosylation of Recombinant Therapeutic Glycoproteins in Plant Systems

Biljana Balen orcid.org/0000-0002-3745-9865 ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Marijana Krsnik-Rasol ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

Abstract

Transgenic plants have recently emerged as a promising system for low-cost and large-scale production of therapeutic proteins. Despite many advantages plants offer as hosts for expression of recombinant proteins, there are certain limitations caused by major structural differences between plant and mammalian N-linked glycans. Thus, efforts are being made in order to eliminate, or at least minimize, plant-specific N-glycosylation as well as to supplement the plant endogenous system with human glycosyltransferases to obtain »humanized« non-immunogenic N-glycans on plant-made pharmaceuticals aimed for human therapy.

Keywords

N-glycosylation; transgenic plants; glycosyltransferases; sialic acid; recombinant protein; therapeutic glycoproteins

Hrčak ID:

30429

URI

https://hrcak.srce.hr/30429

Publication date:

15.3.2007.

Article data in other languages: croatian

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