Original scientific paper

https://doi.org/10.5562/cca3912

Micro-Raman Spectroscopy for Detection of Label-Free and Oil Red O Labeled PEGylated Nanoliposomes in hCmec/D3 Cell Internalization Studies

Filip Gorachinov ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Petre Makreski ; Ss Cyril and Methodius University in Skopje, Faculty of Natural Sciences and Mathematics, Institute of Chemistry, Arhimedova 5, 1000 Skopje, Republic of North Macedonia
Dushko Shalabalija ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Ljubica Mihailova ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Maja Simonoska Crcarevska ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Marija Glavas Dodov ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Katerina Goracinova ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia
Gligor Jovanovski ; Macedonian Academy of Science and Arts, Research Center for Environment and Materials, Bul. Krste Misirkov 2, 1000 Skopje, Republic of North Macedonia
Nikola Geskovski orcid.org/0000-0002-2073-5632 ; Ss Cyril and Methodius University in Skopje, Faculty of Pharmacy, Institute of Pharmaceutcal technology, Majka Tereza 47, 1000 Skopje, Republic of North Macedonia

Supplements: cca3912-supplement.pdf

Abstract

Rapid development of nanomedicines necessitates advancement in internalization techniques which can accurately distinguish between the complex environments of cells and nanocarriers. Internalization (or endocytosis) studies of oil red O labeled and label-free PEGylated-lecithin/cholesterol nanoliposomes was performed using micro-Raman spectroscopy. The C.O stretching vibrations and CCH scissoring bendings of naphthalene ring around 1225 cm.1 as well as the N=N stretching vibrations at 1377 cm.1 are prominent peaks absent from the label-free spectra which can be used for detection of internalized oil red O labeled nanoliposomes. Suitability of oil red O as a liposome marker was confirmed by stability studies of the incorporated dye and automated fluorescence cell counting. The C.C stretching region with a prominent wide band centered at 1080 cm.1 indicative of larger gauche conformer content typical for the lecithin-cholesterol nanoliposomes and the strong maximum at 980 cm.1 associated with O.C.C.N+ stretching vibrations of the liposome polar head groups are important for studying label-free nanoliposome cell internalization.

Keywords

PEGylated nanoliposomes; oil red O labeled PEGylated-nanoliposomes; cell internalization studies; micro-Raman spectroscopy

Hrčak ID:

305557

URI

https://hrcak.srce.hr/305557

Publication date:

5.7.2023.

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