Review article

https://doi.org/10.15255/KUI.2024.018

Hydrogenases – Types, Sources, Properties, and the Potential for Their Application

Ana-Katarina Marić ; University of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, HR-10 000 Zagreb, Croatia
Anđela Radan ; University of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, HR-10 000 Zagreb, Croatia
Nevena Milčić orcid.org/0000-0003-0712-2417 ; University of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, HR-10 000 Zagreb, Croatia
Martina Sudar orcid.org/0000-0002-6734-1024 ; University of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, HR-10 000 Zagreb, Croatia
Zvjezdana Findrik Blažević orcid.org/0000-0002-5312-8951 ; University of Zagreb Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, HR-10 000 Zagreb, Croatia *

* Corresponding author.

Abstract

Hydrogenases are a group of versatile metalloenzymes that catalyse the reversible transformation between hydrogen gas and its constituent protons and electrons. These enzymes have gained attention more recently due to their ability to use molecular hydrogen as a substrate in the reactions they catalyse, as well as their potential to synthesise hydrogen, an important biofuel. Although the existence of hydrogenases has been known since the 1930s, current research has not yet made them viable on the industrial scale. They are explored mostly from the biochemical point of view, which is very important, but there is a lack of research on how to adapt these enzymes for real industrial-scale processes. Only a few studies address this gap. Therefore, in this brief literature review, we provide an overview of what is known about hydrogenases. We explore their background, classification, and phylogeny, highlighting their presence in many different sources in nature, such as bacteria, archaea, and certain eukaryotes. We also discuss key factors influencing their activity, along with their advantages and disadvantages. Furthermore, we summarise the methods available for determining their activity and emphasise the need for standardised units to ensure comparability of all data available in the literature. Finally, we discuss their potential applications, particularly in hydrogen production and synthetic reaction pathways for coenzyme regeneration, emphasizing the critical importance of these aspects in the investigation of hydrogenases.

Keywords

hydrogenase; coenzyme regeneration; hydrogen production; hydrogenase classification; hydrogenase properties

Hrčak ID:

326147

URI

https://hrcak.srce.hr/326147

Publication date:

16.1.2025.

Article data in other languages: croatian

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