Original scientific paper
Deciphering the Bioactive Potential of Four Malaysian Litsea Species for Antioxidant, Antifungal, and Antibiofilm Activities
Audrey Ashleeynus Allhin
; Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak, Malaysia
Wan Mohd Nuzul Hakimi Wan Salleh
orcid.org/0000-0003-1408-229X
; Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak, Malaysia; Center of Biodiversity and Conservation, Faculty of Science and Mathematics, Universiti Pendidikan, Perak, Malaysia
Abubakar Siddiq Salihu
; Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, Perak, Malaysia; Department of Pure and Industrial Chemistry, Faculty of Natural and Applied Sciences, Umaru Musa
Mohd Hafiz Arzmi
orcid.org/0000-0002-9470-6412
; Kulliyyah of Dentistry, International Islamic University Malaysia, Pahang, Malaysia; Melbourne Dental School, The University of Melbourne; Oxford RMC Science Ltd. University of Oxford, England, United Kingdom
Nurunajah Ab Ghani
; Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA, Puncak Alam, Selangor, Malaysia; Faculty of Applied Sciences, Universiti Teknologi MARA, Selangor, Malaysia
Abstract
This study evaluates the antioxidant, antifungal, and antibiofilm activities of methanolic leaf extracts from four Litsea species (L. glauca, L. fulva, L. rubicunda, and L. tomentosa), highlighting their potential as natural therapeutic agents. The antioxidant activity was assessed using the β-carotene/linoleic acid bleaching assay, DPPH radical scavenging assay, and total phenolic content (TPC) determination. Among the species, L. glauca exhibited the highest antioxidant activity, with an 85.2% inhibition of β-carotene bleaching, a DPPH IC50 value of 126.2 μg/mL, and the highest TPC of 130.1 mg GA·g-1, demonstrating a strong correlation between phenolic content and antioxidant efficacy. Antifungal activity was evaluated against Candida albicans, Candida lusitanae, and Candida auris, with L. tomentosa showing the strongest activity, achieving MIC and MFC values as low as 62.5 μg/mL against C. albicans. Antibiofilm activity was also notable, particularly for L. tomentosa and L. rubicunda, which significantly inhibited biofilm formation in C. lusitanae (70.0% and 65.5%, respectively) and C. auris (67.2% and 65.8%, respectively). These findings underscore the potential of Litsea species as sources of bioactive compounds for medicinal and industrial applications, warranting further research to isolate active constituents, elucidate mechanisms of action, and validate efficacy in vivo.
Keywords
Lauraceae, Litsea, antibiofilm, antifungal, antioxidant
Hrčak ID:
333058
URI
Publication date:
19.5.2026.
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