Original scientific paper
Cytotoxic, antibacterial and antioxidant activity of the methanolic extract of speedwells (Veronica officinalis L.)
Emir Horozić
; Faculty of Technology, University of Tuzla
*
Lejla Mekić
Suada Cipurković
Lamija Kolarević
Darja Husejnagić
Merima Ibišević
Enida Karić
Ermina Cilović-Kozarević
orcid.org/0000-0002-4549-0109
Maja Pođanin
Sanja Brekalo-Lazarević
Melisa Šehić
Edina Huseinović
* Corresponding author.
Abstract
Speedwells (Veronica officinalis L.) is a plant species used in traditional medicine for the preparation of teas, tinctures and other preparations for the treatment of diseases of the skin, respiratory and digestive organs. In this paper, the biological activity of the methanolic extract of the speedwells was examined. To assess the cytotoxic potential, a tetrazolium salt reduction (MTT) viability assay was performed. The experiments were carried out on 3 human cell lines: lung carcinoma (H460), cervical adenocarcinoma (HeLa) and colorectal carcinoma (HCT116). Antimicrobial potential was tested using the diffusion technique on three bacterial strains: S. aureus, E. faecalis and S. enterica. Inhibition of free radicals was tested using the ABTS and DPPH methods, and the reduction potential of the extract of the speedwells was confirmed by the FRAP method. The treatment of HeLa, H460, and HCT116 cell lines with the methanolic extract of speedwells demonstrated a dose-dependent decrease in cell growth. The extract showed a high inhibition of the growth of S. aureus but also a complete absence of activity in the case of E. faecalis. A high efficiency of inhibition of DPPH and ABTS radicals, as well as reducing ability, was recorded.
Keywords
polyphenols; flavonoids; cytotoxicity; antibacterial activity; antioxidant potential
Hrčak ID:
335774
URI
Publication date:
2.5.2025.
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