Original scientific paper
https://doi.org/10.5599/jese.3157
Poly(asparagine)-modified duplex stainless steel composite carbon paste electrode for selective electrochemical detection of dopamine
Shashanka Rajendrachari
; Department of Chemistry, Global Academy of Technology, Bangalore-560098, Karnataka, India
*
Hareesha Nagarajappa
; Department of Chemistry, Soonchunhyang University, Asan 31538, Republic of Korea
Varun Donnakatte Neelalochana
; Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano, 77, Trento 38123, Italy
Rakshitha Gattavadipura Shivaraju
orcid.org/0009-0000-1984-8587
; Department of Chemistry, SJCE, JSS Science and Technology University, Mysuru-570 006, Karnataka, India
Ersin Demir
orcid.org/0000-0001-9180-0609
; Department of Analytical Chemistry, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar 03100, Türkiye
Santhy Antherjanam
; Department of Chemistry, Amrita Vishwa Vidyapeetham, Amritapuri, Kollam 690525, India
Kazim Erden Karaoglanli
; Department of Biotechnology, Faculty of Sciences, Bartin University, Bartin 74110, Türkiye
* Corresponding author.
Abstract
Dopamine (DA) is a vital neurotransmitter used in clinical diagnostics and neurochemical studies. In this article, a robust, accurate, and highly sensitive method for determining DA using a duplex stainless steel (DSS)-modified carbon paste electrode (MCPE) and cyclic voltammetry is reported. To improve the sensitivity of the DSS-MCPE, the electrode was polymerized via 10 potential cycles using asparagine, a non-essential amino acid, thereby forming poly(asparagine) on the electrode surface. This polymerized electrode surface acts as a selective barrier to DA and enables the DSS-MCPE to detect DA accurately, even in the presence of interfering molecules such as ascorbic and uric acids, which have overlapping oxidation potentials. Compared with the bare carbon paste electrode (BCPE) and DSS-MCPE, poly(asp)-DSS-MCPE exhibits excellent electrochemical behaviour, a higher oxidation peak current, and improved electron transfer kinetics. The effects of variations in scan rate, pH, and DA concentration on the electrocatalytic behaviour of poly(asp)-DSS-MCPE were investigated. Also, the electrode active surface area was calculated, and the limit of detection, limit of quantification, and number of electrons and protons involved in electrochemical reactions were determined. The ease of fabrication, cost-effectiveness, and robust performance of poly(asp)-DSS-MCPE make it a promising electrocatalyst for detecting DA and other bioactive molecules, without interference from interfering molecules.
Keywords
Catecholamine; voltametric determination; mechanical alloying; asparagine polymer
Hrčak ID:
344839
URI
Publication date:
23.2.2026.
Visits: 232 *