Short communication, Note
https://doi.org/10.18054/pb.v127i3-4.34742
Partial decomposition of Tartary buckwheat and grape seed flavonoids during the preparation of pasta
Blanka Vombergar
; The Education Centre Piramida Maribor, Maribor, Slovenia
Aleksandra Golob
; Biology Department, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
Martin Šala
; National Institute of Chemistry, Ljubljana, Slovenia
Ivan Kreft
orcid.org/0000-0003-3339-8476
; Biology Department, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia; Nutrition Institute, Ljubljana, Slovenia
*
Nataša Pem Rebernik
; The Education Centre Piramida Maribor, Maribor, Slovenia
Mateja Germ
; Biology Department, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
* Corresponding author.
Abstract
Background and purpose: Grain of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) is rich in phenolic substances, especially flavonoid rutin. The grains are used in Asia and Europe mainly to make pasta products. Grape (Vitis vinifera L.) processing, mainly for wine, results in byproducts, including seeds, rich in secondary plant metabolites. Main grape seed flavonoids are catechin and epicatechin. There is a need for applying grape seed flavonoids in human nutrition. As is known, flavonoids could be subjected to metabolic changes during dough processing.
Materials and methods: Combined Tartary buckwheat/vinegrape pasta was produced in pasta machine. Starting seed flours and pasta were analyzed by LC-MS performed on UltiMate 3000 UHPLC system, coupled with a triple quadrupole/linear ion trap mass spectrometer. Methanol (Chromasolv LC-MS grade, Fluka, Switzerland) and purified water were used for the preparation of mobile phases, and formic acid (Fluka) was used as modifier. An analytical HPLC column Hypersil GOLD Aq, Thermo 150x2.1, 3 μm, was used with the flow rate of 0.3 mL min–1. A mobile phase consisting of methanol and water, both modified with 0.1% formic acid was used throughout the work.
Results: In the present research, it was established that in the dough from the mixture of Tartary buckwheat and grape seed flours it is decomposed a considerable part of Tartary buckwheat flavonoid rutin, and grape seed flavonoids catechin and epicatechin.
Conclusion: Procedures for minimizing the loss of flavonoids during the manipulation of combined Tartary buckwheat and grape seed flour dough and preparing pasta are needed.
Keywords
polyphenols; flour; grain; rutin; catechin
Hrčak ID:
347136
URI
Publication date:
13.5.2026.
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