Original scientific paper
https://doi.org/10.3325/cmj.2025.66.345
The role of miR-145, miR-200c, and miR-21 in distinguishing between hyperplastic and adenomatous colon polyps and colorectal cancer prediction
Esra Guzel Tanoglu
; Department of Molecular Biology and Genetics, Institution of Hamidiye Medical Sciences, University of Health Sciences Turkey, Istanbul, Turkey
Alpaslan Tanoglu
; Division of Gastroenterology, Department of Internal Medicine, Bahçeşehir University, Istanbul, Turkey
*
Samet Ebinc
; Department of Molecular Biology and Genetics, Institution of Hamidiye Medical Sciences, University of Health Sciences Turkey, Istanbul, Turkey
Ismail Yılmaz
; Department of Pathology, Sultan Abdulhamid Han Training and Research Hospital, University of Health Sciences Turkey, Istanbul, Turkey
Muhammed Fevzi Esen
; Department of Health Information Systems, Institution of Hamidiye Medical Sciences, University of Health Sciences Turkey, Istanbul, Turkey
* Corresponding author.
Abstract
Aim To assess whether miRNA expression can be a bio
marker for distinguishing between hyperplastic and ade
nomatous polyps and colon cancer tissues.
Methods The study involved 40 colon adenocarcinoma
(CA) tissue samples, 40 low-grade adenomatous (A) pol
yps, and 40 hyperplastic (HP) polyps. The samples were
formalin-fixed and paraffin-embedded. Quantitative real
time polymerase chain reactions were used to determine
the expression levels of miR-21, miR-200c, and miR-145.
Results miR-145 expression was significantly lower in
the CA than in both the A and HP groups (P = 0.012 and
P = 0.004, respectively) and in the A than in the HP group
(P = 0.028). miR-200c expression was higher in the CA than
in the A (P = 0.008) and HP groups (P = 0.009), while no
difference was observed between the A and HP groups
(P = 0.624). miR-21 showed a stepwise increase across the
groups, with the highest levels in the CA, followed by the
A and HP groups (P < 0.001 for both CA vs A and CA vs HP;
P = 0.037 for A vs HP).
Conclusion The study revealed dysregulated expression
of miR-145 and miR-21 in colon polyps and cancer tissue,
showing that these miRNAs may be used to distinguish
between hyperplastic and adenomatous polyps and pre
dict colorectal cancer.
Keywords
Hrčak ID:
352548
URI
Publication date:
22.10.2025.
Visits: 0 *