Original scientific paper
Human Umbilical Cord Bloodderived Stromal Cells Suppress Xenogeneic Immune Cell Response In Vitro
Lei Hao
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Cheng Zhang
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Xing-hua Chen
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Zhong-min Zou
; Department of Chemical Defense and Toxicology, Third Military Medical University, Chongging, People's Republic of China
Xi Zhang
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Pei-yan Kong
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Xue Liang
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Lei Gao
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Xian-gui Peng
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Ai-hua Sun
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Qing-yu Wang
; Department of Hematology, Xingioa Hospital, Third Military Medical University, Chongging, People's Republic of China
Abstract
Aim To explore immunological properties of human umbilical
cord blood-derived stromal cells (hUCBDSC) and
their effect on xenogeneic immune cells in vitro.
Methods Immunological phenotype of freshly isolated
and cryopreserved hUCBDSCs was evaluated by flow cytometry.
Xenogeneic splenic T-cells were stimulated by
phytohemaglutinin A (PHA) or dendritic cells in the absence
or presence of hUCBDSCs. T-cell proliferation was
measured by cell counting kit-8 after 7-day incubation. The
proportion of apoptotic cells and CD4+CD25+Foxp3+ regulatory
T-cells (Tregs) was determined in T-cells activated
by PHA in the absence or presence of hUCBDSCs by flow
cytometry. Phenotype of dendritic cells, cultured alone or
with hUCBDSCs, was analyzed by flow cytometry.
Results Levels of immune molecule expression on freshly
isolated hUCBDSCs were as follows: human leukocyte
antigen-I (HLA-I) (84.1 ± 2.9%), HLA-II (1.6 ± 0.3%), CD80
(0.8 ± 0.1%), CD86 (0.8 ± 0.1%), CD40 (0.6 ± 0.1%), and CD40L
(0.5 ± 0.1%), which was not influenced by cryopreservation.
T-cell proliferation in the presence of hUCBDSCs was significantly
lower than that of positive control. The coculture
led to a 10-fold increase (from 1.2 ± 0.3% to 12.1 ± 1.4%,
P < 0.001) in the proportion of CD4+CD25+Foxp3+ regulatory
T-cells (Tregs) and a reversion of mature dendritic
cells, as indicated by the down-regulation of major histocompatibility
complex (MHC)-II molecule (49.3% vs
25.9%, P = 0.001), CD80 (47.2% vs 23.3%, P = 0.001), and
CD86 (40.6% vs 25.1%, P = 0.002). When subjected to annexin
V binding and propidium iodide uptake assay, the
hUCBDSCs did not show the ability to induce apoptosis of
xenogeneic T-cells.
Conclusion These results demonstrate low immunogenicity
and immunomodulation effect of the hUCBDSCs.
Reversion of mature dendritic cells and increase in Treg
proportion, but not cell apoptosis, can possibly contribute
to the suppression of xenogeneic T-cell proliferation by the
hUCBDSCs.
Keywords
Umbilical cord blood; Stromal cells; Xenogeneic; GVHD
Hrčak ID:
41346
URI
Publication date:
15.8.2009.
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