Original scientific paper
Genetic Diversity in Southeast European Soybean Germplasm Revealed by SSR Markers
Daniela Ristova
; University of Goce Delcev, Faculty of Agriculture, Krste Misirkov bb, 2000 Stip, Macedonia; New York University, Department of Biology, 1009 Silver Building, 100 Washington Square East, New York, NY 10003
Hrvoje Šarčević
; University of Zagreb, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia
Silvio Šimon
; University of Zagreb, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia
Ljupčo Mihajlov
; University of Goce Delcev, Faculty of Agriculture, Krste Misirkov bb, 2000 Stip, Macedonia
Ivan Pejić
; University of Zagreb, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia
Abstract
Breeding material and registered soybean cultivars in Southeast European countries are strongly linked to Western breeding programs, primarily in the USA and Canada. There is little reliable information regarding the source of germplasm introduction, its pedigree and breeding schemes applied. Consequently, use of these genotypes in making crosses to develop further breeding cycles can result in an insufficient level of genetic variability.
The objective of this study was to assess genetic diversity and relationships of 23 soybean genotypes representing several independent breeding sources from Southeastern Europe and five plant introductions from Western Europe and Canada using 20 SSR markers. In total 80 alleles were detected among 28 genotypes with an average of four alleles per locus and an average marker diversity of 0.585. Allele frequency distribution was characterised with a high proportion of alleles at very low frequencies with 11 % of unique alleles. Cluster analysis clearly separated all genotypes from each other assigning them into three major clusters, which largely corresponded to their origin. Results of clustering were mainly in accordance with the known pedigrees.
Keywords
Glycine max; genetic similarity; microsatellites
Hrčak ID:
53004
URI
Publication date:
26.3.2010.
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