Croatica Chemica Acta, Vol. 78 No. 3, 2005.
Original scientific paper
Determination of Mammillaria gracillis N-glycan Patterns by ESI Q-TOF Mass Spectrometry
Biljana Balen
Alina Zamfir
Sergey Y. Vakhrushev
Marijana Krsnik-Rasol
Jasna Peter-Katalinić
Abstract
N-glycans detached from the 42 kDa cellular glycoprotein of in vitro propagated cactus (Mammillaria gracillis Pfeiff.) shoots, callus, hyperhydric regenerants and TW tumour were analyzed by mass spectrometry after in-gel deglycosylation of excised protein bands. Mixtures of N-glycans were shown by the electrospray MS analysis to be highly complex and heterogeneous. Only a low amount of high-mannose N-glycans was present, while the majority of structures were of the complex-type N-glycans. A single N-glycan structure was observed in the shoot sample, which appeared to be in good correlation with previous results. Similarities of assigned structures were revealed in the callus and tumour tissue while the highest number of different oligosaccharide structures was found in the hyperhydric regenerant. The presence of sialic acid as a constituent of N-glycans was indicated by lectin staining, ion exchange chromatography and molecular mapping by mass spectrometry, and could be correlated with the differentiation status of the tissues. Results obtained in this study indicate that different morphological levels can be correlated with the N-glycan composition of cellular glycoproteins.
Keywords
Cactaceae; crown-gall tumour; morphogenesis; N-glycans; sialic acid
Hrčak ID:
58
URI
Publication date:
15.9.2005.
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