Original scientific paper

Free and Bound Sulphur Containing and Other Volatile Compounds from Evergreen Candytuft (Iberis sempervirens L.)

Josip Mastelić
Ivica Blažević
Igor Jerković

Abstract

Volatile fractions of evergreen candytuft, Iberis sempervirens (Brassicaceae), were isolated by simultaneous hydrodistillation-extraction from fresh plant material prior and after autolysis and from dried plant material and were then analyzed using GC and GC-MS. Isothiocyanates, nitriles, aliphatic alcohols, carbonyls, fatty acids, hydrocarbons, terpene compounds, C13-norisoprenoids and phenylpropane derivatives were identified – thirty-nine components in all. Major components of all samples were: 3-butenyl isothiocyanate (17.4–63.3 %), 5-methylthiopentanonitrile (2.2–9.5 %), 4-methylthiobutanonitrile (4.8–6.9 %), allyl isothiocyanate (4.0–6.6 %), 4-methylthiobutyl isothiocyanate (2.9–6.2 %) and 3-methylthiopropyl isothiocyanate (1.3–3.4 %). Oil isolated after autolysis is more complex than the other two oils. It is composed of a large number of compounds. Some of them are identified as O-aglycones such as eugenol, (Z)-3-hexene-1-ol and 1H-indole. O-glycosides of volatile compounds were isolated from fresh plant material and purified by selective extraction and column chromatography. After hydrolysis with b-glucosidase, the liberated volatile aglycones were also analyzed by GC and GC-MS. Nineteen aglycones were identified. The main aglycones identified were: eugenol (15.2 %), 2-phenylethanol (12.9 %), 2-hydroxy-b-ionone (10.6 %), 2-tert-butyl-5-methylphenol (9.2 %) and methyl-2,5-dihydroxybenzoate (4.5 %).

Keywords

Iberis sempervirens L.; evergreen candytuft; Brassicaceae; glucosinolates; O-glycosides; volatile aglycones; isothiocyanates; GC-MS

Hrčak ID:

5804

URI

https://hrcak.srce.hr/5804

Publication date:

13.12.2006.

Visits: 2.579 *