Short communication, Note

https://doi.org/10.2478/v10007-011-0008-4

Simultaneous determination of iron and copper in children's sera by FAAS

SVJETLANA LUTEROTTI ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia
TONČICA VUKMAN KORDIĆ ; HALMED - Agency for Medicinal Products and Medical Devices, Zagreb, Croatia
SLAVICA DODIG ; Children´s Hospital Srebrnjak, Zagreb, Croatia

Abstract

A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L–1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L–1 HCl standard. During method’s optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.

Keywords

iron; copper; children´s serum; single-step determination; FAAS

Hrčak ID:

63379

URI

https://hrcak.srce.hr/63379

Publication date:

1.3.2011.

Article data in other languages: croatian

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