Acta Pharmaceutica, Vol. 61 No. 1, 2011.
Kratko priopćenje
https://doi.org/10.2478/v10007-011-0008-4
Simultaneous determination of iron and copper in children's sera by FAAS
SVJETLANA LUTEROTTI
; University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia
TONČICA VUKMAN KORDIĆ
; HALMED - Agency for Medicinal Products and Medical Devices, Zagreb, Croatia
SLAVICA DODIG
; Children´s Hospital Srebrnjak, Zagreb, Croatia
Sažetak
A new and simple flame atomic-absorption spectrometric (FAAS) method is proposed for simultaneous determination of iron and copper in children's sera. It is based on single-step sample pretreatment (deproteinization with 3 mol L–1 HCl, ratio 1:1) and single-step calibration using 1.5 mol L–1 HCl standard. During method’s optimization a short multifactorial design experiment was used. The proposed method assures accuracy, sensitivity and precision comparable to that of the reference methods. The new approach is simple and time-, labour- and serum-saving, the latter being especially important in pediatric diagnostics.
Ključne riječi
iron; copper; children´s serum; single-step determination; FAAS
Hrčak ID:
63379
URI
Datum izdavanja:
1.3.2011.
Posjeta: 2.713 *