Original scientific paper

Response Surface Methodology Approach for the Synthesis of Ethyl Butyrate

Vitor Cardoso Aragão ; Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil
Maria Rita Alaniz Porto ; Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil
Carlos André Veiga Burkert ; Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil
Susana Juliano Kalil ; Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil
Janaína Fernandes de Medeiros Burkert ; Department of Chemistry, Federal University Foundation of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil

Abstract

Response surface methodology was used to determine optimum conditions for the esterification of ethanol and butyric acid to produce a flavour ester using immobilized lipase. Various reaction parameters including butyric acid concentration, enzyme concentration, temperature and ethanol/butyric acid molar ratio affecting ethyl butyrate production were investigated using a fractional factorial design 24–1. Based on the results from the first factorial design, all of the variables which were significant in the process were selected to be used in a 24 central composite rotatable design (CCRD). The optimum conditions for the enzymatic reaction were obtained at a 90 mM butyric acid concentration using a 7.7 g/L enzyme concentration at 45 °C and the ethanol/butyric acid molar ratio of 1:1 for 3 h. The esterification percentage, under these conditions, was 87 %.

Keywords

ethyl butyrate; immobilized lipase; esterification; optimization; enzymatic synthesis

Hrčak ID:

65627

URI

https://hrcak.srce.hr/65627

Publication date:

21.3.2011.

Article data in other languages: croatian

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