Original scientific paper

Egg immunoglobulins - an alternative source of antibody for diagnosis of infectious bursal disease.

Shanmugasamy Malmarugan ; Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu, India
Muthusamy Raman ; Department of Veterinary Parasitology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India
Sreenivasan Jaisree ; Final Year B.V. Sc Students, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India
Palanisamy Elanthalir ; Final Year B.V. Sc Students, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India

Abstract

The current trends for pursuing research programmes warrant abundant, non-invasive experimentation techniques avoiding the use of laboratory animals have led to a search for alternate sources of commercial antibodies for various immunodiagnostic and prophylactic assays. The present experiment exploited the diagnostic property of egg immunoglobulins (IgY) against infectious bursal disease (IBD) as a novel, alternative approach. The anti-IBD -IgY was harvested by the water dilution method, purified by salt precipitation, characterized by SDS - PAGE and assessed for its purity by immunoelectrophoresis. An average 200 - 250 mg. of immunoglobulins were harvested from an egg with 57-60% recovery of specific immunoglobulins. The anti - IBD-IgY thus harvested was found to possess immunodiagnostic potency as assessed by agar gel precipitation test and counter immunoelectrophoresis for replacing the use of conventional antibody.

Keywords

egg immunoglobulins; diagnostic property; infectious bursal disease; chicken

Hrčak ID:

67057

URI

https://hrcak.srce.hr/67057

Publication date:

20.2.2005.

Article data in other languages: croatian

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