Veterinarski arhiv, Vol. 75 No. 1, 2005.
Izvorni znanstveni članak
Egg immunoglobulins - an alternative source of antibody for diagnosis of infectious bursal disease.
Shanmugasamy Malmarugan
; Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, Tamil Nadu, India
Muthusamy Raman
; Department of Veterinary Parasitology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India
Sreenivasan Jaisree
; Final Year B.V. Sc Students, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India
Palanisamy Elanthalir
; Final Year B.V. Sc Students, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Namakkal, India
Sažetak
The current trends for pursuing research programmes warrant abundant, non-invasive experimentation techniques avoiding the use of laboratory animals have led to a search for alternate sources of commercial antibodies for various immunodiagnostic and prophylactic assays. The present experiment exploited the diagnostic property of egg immunoglobulins (IgY) against infectious bursal disease (IBD) as a novel, alternative approach. The anti-IBD -IgY was harvested by the water dilution method, purified by salt precipitation, characterized by SDS - PAGE and assessed for its purity by immunoelectrophoresis. An average 200 - 250 mg. of immunoglobulins were harvested from an egg with 57-60% recovery of specific immunoglobulins. The anti - IBD-IgY thus harvested was found to possess immunodiagnostic potency as assessed by agar gel precipitation test and counter immunoelectrophoresis for replacing the use of conventional antibody.
Ključne riječi
egg immunoglobulins; diagnostic property; infectious bursal disease; chicken
Hrčak ID:
67057
URI
Datum izdavanja:
20.2.2005.
Posjeta: 1.677 *