Original scientific paper
Isoflavone content and antioxidant properties of soybean seeds
I. Mujić
; University of Bihać, Faculty of Biotechnology, Kulina bana 2, 77 000 Bihać, Bosnia and Herzegovina
Edina Šertović
; University of Bihać, Faculty of Biotechnology, Kulina bana 2, 77 000 Bihać, Bosnia and Herzegovina
Stela Jokić
; University of Josip Juraj Strossmayer in Osijek, Faculty of Food Technology Osijek, Franje Kuhaca 20, 31000 Osijek, Croatia
Z. Sarić
; University of Sarajevo, Faculty of Agricultural and Food Sciences, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and Herzegovina
Vildana Alibabić
; University of Bihać, Faculty of Biotechnology, Kulina bana 2, 77 000 Bihać, Bosnia and Herzegovina
Senka Vidović
; Faculty of Technology, Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
Jelena Živković
; Medical Faculty of Nis, Department of Pharmacy, Bulevar Zorana Dindica 81, 18000, Nis, Serbia
Abstract
The isoflavone content and antioxidant properties of five Croatian soybean seed cultivars from two locations were analysed. The content of total and individual isofavones was determined by high performance liquid chromatography. For determination of antioxidant properties scavenging capacity on DPPHֹ radicals has been applied. The total phenolic content, oil and protein content in soybean cultivars were also determined. Significant differences in the content of individual isoflavones were observed within the soybean cultivars. The total phenol content in soybean cultivars ranged from 87.2 to 216.3 mg GAE/100g of soybean. The total isoflavone content in soybean seeds ranged from 80.7 to 213.6 mg/100g of soybean. The most abundant isoflavone in soybean seeds was genistein. There was statistically significant difference (p < 0.05) among two locations in total and individual isoflavone contents. The highest contents of total isoflavones were found in cultivar “os55-95”. Conversely, cultivars poor in isoflavones also showed low levels of DPPH-radical scavenging activity.
Keywords
isoflavone; soybean seeds; antioxidant activity; total phenolic content
Hrčak ID:
70915
URI
Publication date:
15.7.2011.
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