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Food Technology and Biotechnology, Vol. 50 No. 3, 2012.

Review article

In taberna quando sumus: A Drunkard’s Cakewalk Through Wine Proteomics

Pier Giorgio Righetti orcid id orcid.org/0000-0001-8321-9187 ; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Via Mancinelli 7, IT-20131, Milan, Italy
Alfonsina D'Amato orcid id orcid.org/0000-0002-8147-7235 ; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Via Mancinelli 7, IT-20131, Milan, Italy
Elisa Fasoli ; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Via Mancinelli 7, IT-20131, Milan, Italy
Egisto Boschetti ; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta', Politecnico di Milano, Via Mancinelli 7, IT-20131, Milan, Italy

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Abstract

Analysis of white and red wine trace proteomes via capture with combinatorial peptide ligand libraries (CPLL) is reported here. Most of the alcoholic beverages tested (all of Italian origin) were found to contain only traces of casein (on average from 20 to 60 µg/L, with a detectability of as low as 1 µg/L) and not any grape protein any longer, as they had been fined with bovine casein (surprisingly also red wines for which the typical fining agent is egg albumin). However, analysis of untreated white wine (Recioto, from Garganega grapes in the Veneto region) via CPLL capture indeed permitted to detect close to 100 unique gene products from the grapes, suggesting the possibility of proteotyping grand crus, i.e. those aged, high quality wines that should not be treated with fining agents. Thus the CPLL technique could become a formidable tool for traceability of beverages in particular and of foodstuff in general. For trace protein analysis, a new, most powerful CPLL methodology emerges: capture at pH=2.2 in 0.1 % trifluoroacetic acid (TFA) under the conditions mimicking reversed-phase mechanisms of adsorption.

Keywords

alcoholic beverages; trace proteome; combinatorial peptide ligand libraries; food traceability

Hrčak ID:

86919

URI

https://hrcak.srce.hr/86919

Publication date:

14.9.2012.

Article data in other languages: croatian

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