Original scientific paper

Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method

Elsa Reiner
Goran Šinko
Mira Škrinjarić-Špoljar
Vera Simeon-Rudolf

Abstract

This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the advantages and disadvantages of each and concludes with a proposal for an international agreement between laboratories for the evaluation of a standardized protocol.

Keywords

acetylcholinesterase; butyrylcholinesterase; human erythrocytes; human plasma

Hrčak ID:

884

URI

https://hrcak.srce.hr/884

Publication date:

29.9.2000.

Article data in other languages: croatian

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