Izvorni znanstveni članak
Comparison of Protocols for Measuring Activities of Human Blood Cholinesterases by the Ellman Method
Elsa Reiner
Goran Šinko
Mira Škrinjarić-Špoljar
Vera Simeon-Rudolf
Sažetak
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the advantages and disadvantages of each and concludes with a proposal for an international agreement between laboratories for the evaluation of a standardized protocol.
Ključne riječi
acetylcholinesterase; butyrylcholinesterase; human erythrocytes; human plasma
Hrčak ID:
884
URI
Datum izdavanja:
29.9.2000.
Posjeta: 6.556 *