Original scientific paper

In vitro production of neuraminidase by Clostridium chauvoei (Jakari strain).

Nicodemus Maashin Useh ; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Andrew Jonathan Nok ; Departmenrt of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
Ologunja Joseph Ajanusi ; Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
Emmanuel Oluwadare Balogun ; Departmenrt of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
Sunday Blessing Oladele ; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
King Akpofure Nelson Esievo ; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

Abstract

The production of neuraminidase (sialidase, EC 3.2.1.18) by Clostridium chauvoei (Jakari strain) was investigated and a novel neuraminidase was detected in the bacterium in vitro using a fluorescent substrate. Neuraminidase activity was reproducible and had a linear relationship with the amount of bacteria at lower bacterial concentrations. The relationship was no longer linear at higher bacterial concentrations (3.33×107 to 4.44×107 cfu/ml). The possible role of this enzyme in the pathogenesis of blackleg disease is discussed. It is concluded that the enzyme could be playing the role of spreading the disease in the tissues of infected ruminants. Therefore, the use of neuraminidase inhibitors to manage the disease clinically should be thoroughly investigated.

Keywords

neuraminidase; Clostridium chauvoei; blackleg

Hrčak ID:

68177

URI

https://hrcak.srce.hr/68177

Publication date:

21.8.2004.

Article data in other languages: croatian

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