Veterinary Archives, Vol. 81 No. 3, 2011.
Original scientific paper
Characterization of buffalo, poultry and human rotaviruses in Western India.
Ganesh S. Niture
; Department of Animal Biotechnology, College of Veterinary and Animal Sciences, Parbhani, M.S. India
Aviansh G. Karpe
; KNP College of Veterinary and Animal Sciences, Shirwal, Maharashtra State, India
Minakshi Prasad
; Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences (Erstwhile CCS HAU), Hisar, Haryana, India
Abstract
The present investigation describes the detection of rotaviruses among buffalo, poultry and humans. A total of 83 buffalo, 54 poultry faecal and 81 human stool samples were screened by RNA-PAGE for the presence of rotavirus, of which 6 buffalo (12.5%), 4 poultry (7.84%) and 16 human (20.25%) samples were detected positive. All the buffalo and human rotavirus PAGE positive samples depicted a characteristic group A rotavirus migration pattern (4:2:3:2) of RNA segments, whereas a group D like migration pattern (5:2:2:2) was observed in poultry samples. Out of 26 rotavirus positive samples, all the buffalo (23.07%) rotavirus positive samples showed a long electropherotype, while a short migration pattern was revealed in avian samples (15.38%). Among human samples, the majority of the samples (60%) were long electropherotypes followed by short electropherotypes (40%). Hence, a total of 10 different electropherotypes were identified among the three host species, of which four belonged to buffaloes, one poultry, five human, and one human sample was of mixed infection. RNA-PAGE positive samples were further confirmed for the presence of rotavirus by VP4 & VP7 gene specific RT-PCR. The partial length amplification of the VP4 gene of buffalo and human rotaviruses yielded 856bp and 876bp products, respectively. The VP7 gene of both buffalo and human rotaviruses yielded 1062 bp products. On G genotyping of RT-PCR positive buffalo rotavirus samples, none of the samples revealed any G6, G8, and G10 type specific products. However, P genotyping of the same samples revealed the P[11] genotype in only 2 (33.33%) of the buffalo samples. Among the human rotaviruses, 6 (37.5%) were typed as G[1] genotype but remained untypeable for P genotypes. The VP4 and VP7 genes of avian rotavirus could not be amplified. However, the VP6 gene of all avian rotavirus yielded an amplicon of 493bp . The study reports probably the first ever detection of the group D avian rotavirus in Western India.
Keywords
rotaviruses; buffalo; poultry; humans; India
Hrčak ID:
69412
URI
Publication date:
20.6.2011.
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