Original scientific paper
Synthesis and in vitro cytotoxicity of glycans-capped silver nanoparticles
Luciana Dini
; Department of Biological and Enviromental Science and Technology, University of Salento Via per Monteroni, 73100 Lecce, Italy
Elisa Panzarini
; Department of Biological and Enviromental Science and Technology, University of Salento Via per Monteroni, 73100 Lecce, Italy
Antonio Serra
; Department of Material Science, University of Salento Via per Monteroni, 73100 Lecce, Italy
Alessandro Buccolieri
; Department of Material Science, University of Salento Via per Monteroni, 73100 Lecce, Italy
Daniela Manno
; Department of Material Science, University of Salento Via per Monteroni, 73100 Lecce, Italy
Abstract
Silver nanostructures were successfully synthesized through a simple and "green" method using saccharides as reducing and caping agent. Transmission electron microscopy (TEM) and UV-Vis absorption were used to certify the quality of the silver nanoparticles obtained: firstly, size and dispersion.
In this work Silver NanoParticles (AgNPs) cytotoxicity related to saccharides capping (Glucose and (GlucoseSucrose) was explored in human epithelia cervix carcinoma cells (HeLa). The cells were incubated with increasing AgNPs number/cell and HeLa cells viability was monitored for a period of 48 h compared with the positive and negative controls. We observed that the toxicity increases with incubation time and with AgNPs number/cell. In particular, the different cytotoxic degree of the AgNPs, i.e. AgNP-G are more toxic than AgNP-GS, suggest that the cytotoxic effects are largely depended on the capping agent. The highest concentration of AgNP-G number/cell is able to induce extensive cell death of HeLa cells soon after 1hr of incubation; conversely the lowest concentration of AgNP-GS number/cell, surprisingly, is able to induce cell proliferation.
Keywords
Silver Nanoparticles; cytotoxicity; cell viability; TEM; UV-Vis spectroscopy
Hrčak ID:
71570
URI
Publication date:
15.6.2011.
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