Original scientific paper
Use of Mitochondrial DNA Analyses in Verification of the Lipizzan Horse Pedigre
Mato Čačić
orcid.org/0000-0002-1009-8692
; Croatian Agriculture Agency, Ilica 101, 10000 Zagreb, Croatia
Vlatka Cubric-Curik
; University of Zagreb, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia
Mirjana Baban
; University of J. J. Strossmayer in Osijek, Faculty of Agriculture, Trg sv. Trojstva 3, 31000 Osijek
Zdravko Barać
; Croatian Agriculture Agency, Ilica 101, 10000 Zagreb, Croatia
Ino Curik
; University of Zagreb, Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia
Abstract
The Lipizzan horse, established in 1580 in Lipica, is one of the most famous horse breed in the world. To verify the authenticity of maternal lineages in Lipizzan horses we analyzed maternal segregation of the nucleotide sequence of the mitochondrial control region (D-loop mtDNA) (nt 15476 – 15834) through the pedigree. The analyses were done for 258 Lipizzan horses sampled mainly in Croatia, State stud Lipik (40), State stud Đakovo (59) and private farms (129) as well as in Bosnia and Herzegovina, State stud Vučijak (19). Out of 24 analyzed mare lines we have found 23 different haplotypes. On a very long pedigree, tracing up to 36 generations, we found inconsistency (18.2%) in the maternal segregation. In several cases we were able to reconstruct the history and origin of the pedigree errors. Here, for populations with complex pedigree structure, we demonstrated the usefulness of the joint mtDNA and pedigree segregation analyses in tracing pedigree errors and maintenance of reliable herd book identification.
Keywords
Lipizzan; mitochondrial DNA; pedigree error; maternal lineage
Hrčak ID:
72118
URI
Publication date:
4.10.2011.
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