Original scientific paper

A Lysozyme with Antifungal Activity from Pithecellobium dulce Seeds

Narumon Sawasdipuksa ; Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Zhentian Lei ; Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore 73401, Oklahoma, USA
Lloyd W. Sumner ; Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore 73401, Oklahoma, USA
Ploypat Niyomploy ; Biotechnology Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Polkit Sangvanich ; Research Centre for Bioorganic Chemistry, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand

Abstract

A protein of an apparent molecular mass of 14.4 kDa with antifungal activity was isolated from the seeds of Pithecellobium dulce using extraction with 100 mM Tris-HCl buffer (pH=8.0), precipitation with 80 % ammonium sulfate, and bioassay purification via Resource Q anion exchange chromatography and Superdex 200 gel filtration chromatography. The purified protein was putatively identified by tandem mass spectrometry with Mascot database searching, with the partial amino acid sequences showing a high degree of similarity to chicken egg white lysozyme. This putative plant lysozyme expressed antifungal activity with a rather high thermal stability of up to 80 °C for 15 min (at pH=8.0). It exerted an antifungal action towards Macrophomina phaseolina but displayed no antifungal activity against two other isolates, Phymatotrichopsis omnivora and Fusarium avenaceum.

Keywords

Pithecellobium dulce; seed protein; lysozyme; antifungal activity

Hrčak ID:

75188

URI

https://hrcak.srce.hr/75188

Publication date:

20.12.2011.

Article data in other languages: croatian

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