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Original scientific paper

Utjecaj suhoga zagrijavanja i enzimske hidrolize na strukturu pšeničnoga glutena

Haihua Zhang ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China
Irakoze Pierre Claver ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China
Qin Li ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China
Kexue Zhu ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China
Wei Peng ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China
Huiming Zhou ; State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, PR China


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Abstract

Pšenični je gluten modificiran toplinskom metodom suhoga zagrijavanja pa je ispitan utjecaj topline na enzimsku hidrolizu i strukturu glutena. Nakon 1 sata suhoga zagrijavanja stupanj hidrolize pšeničnoga glutena porastao je s 20 na 30 %. Istodobno je udjel frakcija molekularne mase manje od 1000 Da, određen SE-HPLC metodom, povećan sa 17,6 na 30,7 %. Daljnjom je analizom ispitana struktura toplinski obrađenoga glutena, a FTIR spektroskopijom utvrđeno je da su nastale promjene u sekundarnoj strukturi glutena, tj. proteini nisu više imali izduljeni oblik, nego su oblikovali više β-ploča. Također se smanjila hidratacija i povećala hidrofobnost površine s 56,7 na 74,5 te porastao udjel sulfhidrila s 4,74 na 16,18 μmol/g. Pretpostavlja se da su veći udjel β-ploča i sulfhidrila, a i veća hidrofobnost površine utjecali na povećanje stupnja hidrolize toplinski obrađenoga glutena.

Keywords

gluten pšenice; suho zagrijavanje; FTIR; SE-HPLC; enzimska hidroliza

Hrčak ID:

78998

URI

https://hrcak.srce.hr/78998

Publication date:

27.3.2012.

Article data in other languages: english

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