Original scientific paper

GLUTENIN LOCI VARIABILITY OF CROATIAN WHEAT GERMPLASM

Ivana Rukavina ; Croatian Center for Agrculture, Food and Rural Affairs, Institute for Seeds and Seedlings, Osijek, Croatia
Sonja Marić ; J.J. Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Croatia
Vlado Guberac ; J.J. Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Croatia
Tihomir Čupić ; Agricultural Institute Osijek, Osijek, Croatia
Cornelia Tepper ; Bundessortenamt, Hannover, Njemačka

Abstract

Glutenins loci were used for variability estimation in 50 varieties of hexaploid winter wheat originated from Croatian breeding centres. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium dodecyl sulphate (SDS) was used for determination of high molecular weight glutenins (HMW-GS). Number of allels per loci ranged from 3 at Glu-A1 to 5 at Glu-B1 and Glu-D1, the average number of allels was 4.33. The highest genetic diversity was found at loci Glu-B1 (He=0.687). The most frequent subunit at loci Glu-A1 was 2* (56%). At loci Glu-B1 the most common combination of subunits was 7+8 with 40%, and at loci Glu-D1 5+10 with 68%. The study also defines high quality varieties with largest number of Glu-scores. The results attained from this study allow further development of specific breeding programs for winter wheat quality improvement and improvers creation.

Keywords

wheat; variability; glutenins; SDS PAGE

Hrčak ID:

94627

URI

https://hrcak.srce.hr/94627

Publication date:

17.12.2012.

Article data in other languages: croatian

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