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Croatica Chemica Acta, Vol. 77 No. 4, 2004.

Original scientific paper

Study of Chromatographic Enantioseparation of the Esters of N-Dinitrobenzoyl (N-DNB) and N-Benzoyl (N-B) α-Amino Acids on Novel Chiral Stationary Phases Containing Structurally Matching N-DNB and N-B-α-AA Amides in the Chiral Selector

Biljana Zafirova ; Ruđer Bošković Institute, P. O. Box 180, Bijenička cesta 54, 10002 Zagreb, Croatia
Goran Landek ; Ruđer Bošković Institute, P. O. Box 180, Bijenička cesta 54, 10002 Zagreb, Croatia
Darko Kontrec ; Ruđer Bošković Institute, P. O. Box 180, Bijenička cesta 54, 10002 Zagreb, Croatia
Vitomir Šunjić ; Ruđer Bošković Institute, P. O. Box 180, Bijenička cesta 54, 10002 Zagreb, Croatia
Vladimir Vinković ; Ruđer Bošković Institute, P. O. Box 180, Bijenička cesta 54, 10002 Zagreb, Croatia

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Abstract

Enantioseparation ability and enantiopreference of chiral stationary phases CSP 1–CSP 3, containing a terminal N-3,5-dinitrobenzoyl (N-DNB) unit, and CSP 4, containing a terminal N-benzoyl (N-B) unit, are studied. Separation factors (α) for the two sets of test racemates (TR) that structurally match the chiral selector of these CSPs have been determined. The first set consists of seven N-DNB α-amino acid isopropylesters (TR 1A–TR 7A), and the second one of their N-B analogues (TR 1–TR 7). The best enantioseparation (αaverage 1.27) is obtained when π-acceptor DNB unit is present in both TR and CSP. One π-acceptor unit, either in the analyte or in CSP, suffices for efficient enantioseparation (αaverage 1.19). Interaction between π-neutral units in the CSP and test racemate does not afford effective enantioseparation (αaverage 1.03). Using (S)-enantiomers of all TRs as standards, CD detection has revealed regular preference of the CSPs for the enantiomers containing amino acid amide of the same absolute configuration. The possible origin of such enantiopreference is discussed.

Keywords

π –π interactions; chiral stationary phases; Pirkle-type; electron-deficient groups

Hrčak ID:

102978

URI

https://hrcak.srce.hr/102978

Publication date:

30.11.2004.

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