Original scientific paper

GLIADIN LOCI COMPOSITION ANALYSIS OF CROATIAN HEXAPLOID WHEAT GERMPLASM

Ivana Rukavina ; Croatian Centre for Agriculture, Food and Rural Affairs – Institute for Seed and Seedlings
Sonja Marić ; Faculty of Agriculture University of Osijek
Vlado Guberac ; Faculty of Agriculture University of Osijek
Sonja Petrović ; Faculty of Agriculture University of Osijek
Tihomir Čupić ; Agricultural Institute Osijek
Cornelia Tepper ; Bundessortenamt, Hannover, Njemačka

Abstract

The composition of ω – gliadins was identified and analyzed in 50 varieties of
hexaploid winter wheat (Triticum aestivum ssp. vulgare L.) originated from the
Republic of Croatia. Polyacrylamide gel electrophoresis (PAGE) in presence of sodium
dodecyl sulfate (SDS) was used for determination of subunits at Gli-B1 i Gli-D1
genloci. The most common combination of subunits at genloci Gli-B1 was 63+67 with
frequency of 64%, then subunit 66 with frequency of 16% and null allele (N) with
frequency of 14%. At Gli-D1 loci the most frequent subunit was 55 with presence at
94% in tested varieties. Number of allels per loci was from 3 (Gli-D1) to 7 (Gli-B1),
high genetic diversity (He = 0,557) and high PIC (0,529) were estimated at loci Gli-B1,
while significantly lower genetic diversity was estimated at loci Gli-D1 (He = 0,114)
and PIC (0,110).

Keywords

hexaploid wheat; variety; ω – gliadins; subunits; SDS PAGE

Hrčak ID:

103343

URI

https://hrcak.srce.hr/103343

Publication date:

28.3.2013.

Article data in other languages: croatian

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