Original scientific paper

Optimisation of algae Euglena gracilis cultivation conditions for vitamin A and vitamin E production

Tonči Rezić ; Prehrambeno – biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska
Marina Bujić ; Prehrambeno – biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska
Božidar Šantek ; Prehrambeno – biotehnološki fakultet, Pierottijeva 6, 10000 Zagreb, Hrvatska

Abstract

In this research, cultivation of algae Euglena gracilis was studied in order to produce vitamin A and vitamin E. The optimization of medium composition and cultivation conditions, in Erlenmeyer flasks on laboratory shaker, was done to define the most appropriate parameters for vitamin production in the stirred tank bioreactor. During cultivation of E. gracilis in the stirred tank bioreactor two-step process was examined. In the first step photoheterotrofic cultivation on the Hutner medium was done and in the second step photomixotrophically cultivation on the limited carbon source medium. In the two step process following bioprocess efficiency parameters were observed: productivity for vitamine A (PrA =
0.07 mg/Lh) and vitamin E (PrE= 0.27 mg/Lh) production as well as vitamin A (YA = 6.53 mg/L) and vitamin E (YE = 26.26 mg/L) yield. High vitamin E yield indicate potential of two-step processes for this vitamin industrial scale production.

Keywords

Euglena gracilis; vitamin A; vitamin E; photomixotrophic cultivation

Hrčak ID:

105658

URI

https://hrcak.srce.hr/105658

Publication date:

30.6.2013.

Article data in other languages: croatian

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