Agriculture, Vol. 19 No. 2, 2013.
Other
INFLUENCE OF DIETARY SELENIUM SUPPLEMENTATION OF EWES ON PRODUCTION TRAITS, ANTIOXIDANT STATUS AND METABOLIC PROFILE OF LAMBS
Josip Novoselec
; J.J. Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Croatia
Abstract
The aim of this study was to determine the effect of dietary selenium supplementation (organic, inorganic) of high pregnant ewes on the production traits of lambs, the concentration of selenium in the blood of ewes and their lambs, indicators of antioxidant status in the blood of ewes and their lambs, the metabolic profile of ewes and their lambs and concentrations of thyroid hormones. Ewes were in the last third of pregnancy, the average age of four years, healthy and in good condition, divided into three groups of 10 animals. The research lasted 4 months respectively, 2 months with ewes during high pregnancy, 2 months with ewes during lactation and on their lambs during suckling period. Ewes ration from control group one was composed from 300 g/day/animal feed mixture without addition of selenium and 150 g/day/animal barley and alfalfa hay that they had ad libitum. Feed mixture from second group of ewes was supplemented with 0.3 mg/kg organic form of selenium (Sel-Plex®), and feed mixture from third group with the same amount inorganic form of selenium (sodium selenite). Selenium supplementation of ewes feed mixture did not significantly influence on the production traits of their lambs postpartum. Selenium supplementation of ewes and their lambs had influence on a significant (P<0.01; P<0.05) increase in the concentration of selenium, GSH-Px and SOD in whole blood compared to control group of ewes. Organic selenium supplement had a more significant impact on the increase in concentration of selenium and GSH-Px in the blood. In the ewes and lambs blood was determined decrease of MDA with increasing concentrations of selenium in the blood. Generally, the selenium supplementation led to an increase (P<0.05) in the number of WBC and lymphocytes in the blood of ewes and lambs. Also, the increase in the number of RBC, HGB content and MCV in lambs and MCH as well as MCHC in ewes that had a selenium supplement in feed mixture were determined. Concentration of Ca, Cl, Na and Fe significantly increased in the blood of supplemented ewes. Addition of selenium in feed mixture of ewes had influence on significant increase of serum urea and triglycerides and increasing trend of glucose. In the lambs was determined significant decrease in cholesterol, triglycerides, globulin, HDL, and HCO3 -, a significant increase in urea, albumin and LDL levels with the addition of selenium in feed mixture. Inorganic source of selenium influenced on a significant increase of pO2 in the blood of ewes and increasing trend in lambs. Strong ion difference (SID) and the z-value in the blood of lambs, average age of 23 days, were significantly decreased with the addition of inorganic selenium in the feed mixture. Inorganic selenium supplement in the feed mixture of pregnant ewes influenced a significant increase in the anion gap. It was determined a slight increase in activity of AST and LDH and a decrease in serum CK of ewes supplemented with selenium, while in the lambs organic and inorganic selenium supplements influenced on a significant decrease of AST, GGT and CK. Selenium supplementation of high pregnant ewes had influence on the growth of the concentration of T3 and T4 in the blood. Inorganic selenium supplement in lactating ewes influenced a significant increase in concentration of T3. In the lambs, average age 23 days, selenium supplement had influence increase of concentrations of T3 and T4, while aged 63 days had influence on increase T3 and decrease T4. The highest concentration of selenium in milk was determined in ewes that had supplement of organic selenium in the rations. A significant positive correlation between selenium concentration and GSH-Px in the blood of ewes and lambs was determined with the addition of organic selenium in feed mixture. Also, positive correlation was determined between the concentration of selenium in whole blood of ewes and their lambs, which was significant with supplementation of organic selenium. Also, positive correlation between GSH-Px, SOD and MDA was determined in whole blood of ewes and lambs that was stronger in organic compared to inorganic selenium supplement in feed mixture of ewes and lambs. Supplementation of ewes with organic selenium was significantly increased its concentration in the blood of ewes and lambs, and had influence on increase of GSH-Px, which is more reliable indicator in monitoring the supply of body with selenium.
Keywords
ewes; lambs; selenium; production characteristic; antioxidant status; metabolic profile; thyroid gland
Hrčak ID:
112554
URI
Publication date:
20.12.2013.
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