Original scientific paper
USING POLYETHYLENE GLYCOL (6000) FOR DEACTIVATION OF TANNINS IN GRAZING TREE LEAVES GROWN IN EASTERN LIBYA
Ibrahim Salehi Milad
; Omer Al-Mukhtar University, Faculty of Agriculture, Department of Animal production, El-Bieda, Libya
Azzooz O. A. K.
; Omer Al-Mukhtar University, Faculty of Veterinary medicine, El-Bieda, Libya
M. S. I. Ben – Amer
; Omer Al-Mukhtar University, Faculty of Veterinary medicine, El-Bieda, Libya
Abstract
This study was conducted to investigate the effect of different levels of Polyethylene glycol (PEG) on in vitro gas production of some tree leaves grown in Eastern Libya. The samples used were Ceratonia siliqua, Pistacia lentiscus, and Acacia cyanophylla. They were incubated anaerobically with rumen liquor from growing male sheep equipped with a permanent cannula. Cumulative gas production was measured after 3, 6, 12, 24, 48 and 72 hours from the incubation of samples with rumen liquor. PEG was added at levels 15, 30 and 45 mg per 0.2 g dry matter. The chemical analysis showed that the crude protein (%) was 8.2, 9.5, 13.4, for P. lentiscus, C. siliqua and A. cyanophylla, respectively, and that of the ether extracts were nearly the same in all the studied samples. The NDF contents were ranged between 44.5% for C. siliqua and 37% for A. cyanophylla with P. lentiscus lie in the middle (39.8%). The percentages of condensed tannins were 25.4, 21.5 and 4.1 for A. cyanophylla, P. lentiscus and C.siliqua respectively. The average cumulative gas production (ml/0.2 g DM) after 48h of incubation was higher (P<0.05) for C. siliqua then P. lentiscus followed by A. cyanophylla (22.9, 12.6 and 8.2) respectively. Addition of Polyethylene glycol (15, 30 and 45 mg PEG/0.2 g DM) increased (P<0.05) the cumulative gas production compared with control (13.5, 16.4 and 20 vs. 8.4). The current study concluded that PEG can be used to alleviate the undesirable effects of anti-nutritional Polyphenols found in some grazing tree leaves.
Keywords
In vitro gas production; PEG; anti-nutritional compounds
Hrčak ID:
128022
URI
Publication date:
2.10.2014.
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