Biochemia Medica, Vol. 17 No. 1, 2007.
Original scientific paper
Alkaline phosphatase isoenzymes in children with respiratory disease
Slavica Dodig
Jadranka Demirović
Žaneta Jelčić
Darko Richter
Ivana Čepelak
Miljenko Raos
Rajka Petrinović
Kornelija Kovač
Nina Peruško Matasić
Abstract
Aim: To present the electrophoretic pattern of alkaline phosphatase (ALP) isoenzymes in serum of infants and children exhibiting increased total ALP catalytic activity in the course of acute respiratory disease.
Subjects and methods: Results obtained in 21 children (17 of them infants), including 13 male and eight female children aged 2 months to 8 years, hospitalized for respiratory diseases are presented. Total ALP catalytic activity was determined and electrophoretic separation of ALP isoenzymes was performed in children's sera.
Results: Increased total ALP catalytic activity (range, 528-5622 U/L) during hospital stay was recorded in eight (38.1%) children. A typical picture of be-nign transient hyperphosphatasemia (TH), which implies the occurrence of fast anodal fraction (faster than hepatic fraction) and near-cathode fraction (faster than bone fraction), was recorded in five children. The placental-like isoenzyme was detected in two children. Expression of bone fraction and placental-like fraction was recorded in a rachitic child. Prehepatic ALP was expressed in two children, and hepatic ALP isoenzyme in one child.
Conclusion: Acute respiratory disease in infants and children may entail transient increase in the ALP catalytic activity with the occurrence of various isoenzyme bands such as fast anodal and near-cathode fraction (in TH), prehepatic fraction and placental-like fraction. TH is verified when total ALP activity has decreased and returned to reference intervals. In this case, no additional testing is required.
Keywords
alkaline phosphatase; child; infant; hyperphosphatasemia; isoenzymes; respiratory diseases
Hrčak ID:
12918
URI
Publication date:
7.6.2007.
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