Original scientific paper

Lipid peroxidation in dogs naturally infected with Babesia canis canis.

Martina Crnogaj ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Kiš ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Nada Kučer ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Iva Šmit ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Iva Mayer ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Mirna Brkljačić ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Jelena Selanec ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Vladimir Mrljak ; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Abstract

The possible role of oxidative stress in the pathogenesis of infections caused by parasites has been an active area of research in recent years. The aim of this study was to assess changes in activity concentrations of malondialdehyde (MDA), a commonly used biomarker for assessing lipid peroxidation, in dogs infected with Babesia canis canis, after therapy with imidocarb dipropionate, and to compare them with MDA concentrations prior to treatment and to healthy controls. The study was conducted on a sample of 30 dogs suffering from babesiosis and 25 healthy dogs (control group). Blood was taken before treatment, on the first day (all dogs) and on the second, third, and seventh days of the research (affected dogs and ten healthy dogs). Application of imidocarb diproprionate in ten healthy dogs did not affect the values of MDA, so in our study all healthy dogs were used as controls. On the first day of the research the MDA concentrations were significantly increased in dogs with babesiosis (P<0.001) in comparison with the control group. On the seventh day MDA decreased, but was still significantly higher compared to the control group (P<0.001). Concentrations of MDA on the first day were significantly increased in dogs with uncomplicated (P<0.001) and complicated babesiosis (P<0.001) compared to controls. On the seventh day the concentration of MDA was significantly higher in the group of dogs with complicated babesiosis (P = 0.003) than in the uncomplicated group. Furthermore, dogs in the multiple organ dysfunction syndrome group had significantly increased MDA concentrations compared to dogs in the uncomplicated (P = 0.006) and single complication groups (P = 0.025). There was a significant positive correlation between MDA concentration and the outcome of the disease (Tau-b 0.309; P = 0.005). The present study concludes that, on the seventh day, increased lipid peroxidation was still present in the affected group of dogs. The results indicate that oxidative stress could have a possible causative role in the clinical severity of the disease.

Keywords

oxidative stress; malondialdehyde; uncomplicated babesiosis; complicated babesiosis

Hrčak ID:

135265

URI

https://hrcak.srce.hr/135265

Publication date:

23.2.2015.

Article data in other languages: croatian

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