Original scientific paper

https://doi.org/10.2478/v10007-007-0026-4

A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations

PADMARAJAIAH NAGARAJA
SHAILENDRA D. NAIK
ASHWINEE KUMAR SHRESTHA
ANANTHARAMAN SHIVAKUMAR

Abstract

A new, simple and sensitive spectrophotometric method for the determination of some sulfonamide drugs has been developed. The method is based on the diazotisation of sulfacetamide, sulfadiazine, sulfaguanidine, sulfamerazine, sulfamethazine, sulfamethoxazole and coupling with 8-hydroxyquinoline in alkaline media to yield red coloured products, with absorption maximum at 500 nm. The Beer’s law is obeyed from 0.17.0 µg mL1. The limits of quantification and limits of detection were 0.110.18 and 0.030.5 µg mL1, respectively. Intraday precision (RSD 0.10.5%) and accuracy (recovery 97.3100.8) of the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the assay of sulpha drug in the pharmaceutical formulations.

Keywords

sulfonamide drugs; diazotisation; 8-hydroxyquinoline; spectrophotometry; pharmaceutical formulation

Hrčak ID:

13274

URI

https://hrcak.srce.hr/13274

Publication date:

1.9.2007.

Article data in other languages: croatian

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