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Acta Botanica Croatica, Vol. 75 No. 1, 2016.

Original scientific paper

https://doi.org/10.1515/botcro-2016-0003

Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut

Kaitheri Edathil Rachana ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Sudalaimuthu Asari Naganeeswaran ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Thayale Purayil Fayas ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Regi Jacob Thomas ; ICAR-Central Plantation Crops Research Institute (RS), Kayamkulam, Kerala, India
Muliyar Krishna Rajesh ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India

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Abstract

Coconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant cultivar Chowghat Green Dwarf (CGD) by using primers designed based on conserved motifs of the NBS-LRR domain of the date palm. The amplified sequences were cloned, sequenced and characterized. The coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease resistant traits in coconut.

Keywords

coconut; homology modeling; nucleotide binding site-leucine rich repeat type; real time PCR; resistant gene analogue

Hrčak ID:

155267

URI

https://hrcak.srce.hr/155267

Publication date:

1.4.2016.

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