Acta Botanica Croatica, Vol. 29 No. 1, 1970.
Original scientific paper
The Effect of Amitrole on the Fine Structure of Developing Chloroplasts
Branka Vrhovac
; Hrvatska
Mercedes Wrischer
; Hrvatska
Abstract
Growing plants of Zea mays, Phaseolus vulgaris, Elodea canadensis, Elodea densa and Wolffia arrhiza were treated with 10-4 to 10-2M solutions of amitrole and investigated by light and electron microscopy at different intervals after chlorosis appeared in the leaves.
It has been shown that only those plastids were affected by amitrole which were in the stage of differentiation during the treatment.
In addition to changes already known the light microscope revealed characteristic crystals of carotenoids in damaged plastids.
In the electron microscope considerable changes in the ultrastructure have been found. Instead of normal grana there appear long lamellae stuck together. Some of them show a clear striation (period: 12—13 nm) in cross section. In plane sections this striation has been shown to be identical with hexagonally arranged globular particles (diameter 7—8 nm). The possible relations of this structure to the molecular organisation of the normal thylakoids and other details are discussed.
Keywords
Hrčak ID:
155303
URI
Publication date:
31.12.1970.
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