Original scientific paper
The biosynthesis of aflatoxin B1 and G1 during the growth of the mould Aspergillus parasiticus NRRL 2999 in pure and mixed culture at various temperatures of cultivation
S. Duraković
; Zavod za biokemijska inženjerstvo, Prehrambena-biotehnološki fakultet, Zagreb, Hrvatska
O. Pospišl
; Zavod za biokemijska inženjerstvo, Prehrambena-biotehnološki fakultet, Zagreb, Hrvatska
J. Velikonja
; Zavod za biokemijska inženjerstvo, Prehrambena-biotehnološki fakultet, Zagreb, Hrvatska
Abstract
The influence of incubation temperature on the biosynthesis of aflatoxins B1 and G1 has been investigated with the mould A. parasiticus NRRL 2999 during growth in pure culture and in mixed culture containing moulds which do not synthesize aflatoxins. The highest rate of the biosynthesis of aflatoxin B1 was observed at 28 °C after 14 days of cultivation, whereas the biosynthesis of aflatoxin G1 reached its maximum after 21 days. The cultivation temperature of 35 °C favoured a rank mycelium growth, though it inhibited the synthesis of both investigated toxins. After·42 days of cultivation the biomass of the mixed mould culture decreased the concentration of aflatoxin B1 to 60% and the concentration of aflatoxin G1 to 55% of the highest values obtained in the process of biosynthesis. Under equal conditions of cultivation, the biomass of A. parasiticus NRRL 2999 grown in pure culture decreased the concentration of aflatoxin B1 to 75%, and the concentration of aflatoxin G1 to 65% of the highest values obtained.
Keywords
Hrčak ID:
157114
URI
Publication date:
15.1.1985.
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