Original scientific paper

Rhizogenesis of Pumpkin Hypocotyl Explants

Sibila Jelaska ; Hrvatska

Abstract

Hypocotyl fragments of pumpkin were grown on Heller’s synthetic medium or MS-medium with 3°/o glucose and 0.9% Difco Bacto agar and some growth regulators. Heller’s basic medium is not suitable for the growth and development of hypocotyls. On this medium the fragments never show any signs of differentiation and hardly survive the culture period of one month.
Moroever, as shown earlier, IAA with kinetin or 2,4-D with kinetin in Heller’s medium provoke cell divisions resulting in an increase of fresh and dry weight but never in rooting. In a small number of expiants with only 2,4-D at the concentrations of 10-7 and 10-6 very weak rhizogenesis was induced.
The MS-medium has proved to be much more suitable. The medium consisting of MS-salts and sugar only is sufficient for root iduction on the root side of the fragment; for the vigorous root growth, however, the complete MS-medium is necessary. The addition of some substances, e. g. of autoclaved water-melon sap (15% vol.) or autoclaved water-melon sap combined with 2,4-D (3.10-7) further intensifies the vigorous growth of the roots; low concentrations of NAA, high concentrations of IBA and IAA (10-6) with kinetin (3.10-7) show the same effect.

Keywords

Hrčak ID:

157914

URI

https://hrcak.srce.hr/157914

Publication date:

31.12.1974.

Article data in other languages: croatian

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