Original scientific paper

Nutritional and Hormonal Requirements of Meteor Pea Callus Tissue

Mirjana Nešković ; Srbija
Ljiljana Radojević ; Srbija

Abstract

A callus tissue was obtained from an immature embryo of Meteor pea (Pisum sativum cv. Meteor) and its requirements for optimal growth were investigated. The tissue showed rather peculiar responses in respect to the concentration of mineral salts, to the carbohydrate used and to the kind of auxin supplied to the medium. Of the five mineral solutions tested, only two could support growth, in which the concentration of mineral salts was relatively high. It was not possible to show the specific role for any particular element. However, the stimulating effect of any ion was not evident, unless the content of potassium was increased at the same time. Sucrose was the best carbon source, while the tissue could not grow on media containing glucose or fructose. The addition of thiamine was necessary, while nicotinic acid had a beneficial effect. An absolute requirement for auxin was shown. Only the synthetic auxins could stimulate growth of the tissue, while IAA was almost ineffective. Cytokinins and gibberellic acid were not needed, but they considerably increased the weight of the tissue.

Keywords

Hrčak ID:

157954

URI

https://hrcak.srce.hr/157954

Publication date:

31.12.1975.

Article data in other languages: croatian

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