Acta Botanica Croatica, Vol. 38 No. 1, 1979.
Original scientific paper
The application of the replica technique in investigations of physiological groups of bacteria in surface waters
Božidar Stilinović
; Hrvatska
Abstract
The method of replica technique is a new way of determining the presence of physiological groups of bacteria in surface waters. By using the method of replica technique it was established that the percentage of proteolytic and phosphomineralizing bacteria decreased, in relation to the number of saprophytic bacteria on MPA, at the places of the greatest pollution of the river Sava, while at the same time the percentage of amylo- lytic and especially of phosphomobilizing bacteria increased.
The same method was used in examining the influence of temperature on the proteolytic activity of the bacterial strains from river Sava, and it was established that the highest number of bacteria on MPA hydrolyzed gelatine between 22° C and 26° C.
A special advantage of the replica technique method in hydromicrobiology is in the minimal use of laboratory material and in time saving, and it is possible to work out a relatively great number of samples at the same time. The reported method is very simple and feasible in all laboratories.
Keywords
Hrčak ID:
158344
URI
Publication date:
31.12.1979.
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