Original scientific paper

Purification of Belladonna Mottle Virus by Ammonium Sulphate

Đorđe Mamula ; Hrvatska

Abstract

The isolation of belladonna mottle virus (BdMV) by means of salting out with ammonium sulphate (a. s.) is reported. An isolate (BMV-M) of the virus from Yugoslavia was propagated in Datura stramonium and investigated. By adding saturated a. s. solution to clarified sap of infected leaves we tried to precipitate the virus and also plant proteins present in the sap. A part of proteins was precipitated from the suspension when it was 1/3 saturated with the salt. The main quantity of the virus was precipitated at salt concentration which corresponded roughly to 1/2 saturation. This was proved by measuring optical density (light wavelength 260 nm) of precipitate suspensions obtained at different a. s. concentrations and by infectivity test. With respect to the property of the precipitation BdMV shows some similarity with two other tymoviruses, i. e. with cacao yellow mosaic and Andean potato latent viruses which are precipitated from suspension at 1/2 saturation with a. s., too.
Following the treatment with a. s. infectivity as v/ell as integrity of virus particles were preserved greatly, which was confirmed by electron microscope observation, in addition to the assaying methods quoted. Yield and purity of virus preparate obtained were of a satisfactory degree, altogether suggesting that the procedure, because of its simplicity, can be useful in the work with this virus.

Keywords

Hrčak ID:

158360

URI

https://hrcak.srce.hr/158360

Publication date:

31.12.1980.

Article data in other languages: croatian

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