Acta Botanica Croatica, Vol. 39 No. 1, 1980.
Original scientific paper
Plantlet Regeneration from Shoot Tip Culture of Pelargonium zonale Hybrid
Sibila Jelaska
; Hrvatska
Biserka Jelenčić
; Hrvatska
Abstract
Shoot tips (0.5 mm high) of Pelargonium zonale hybrid cvs. ’Career’ and ’Fire flash’ were cultured on modified MS medium: macroelements (Murashige and Skoog 1962), microelements (Nitsch and Nitsch 1970) containing (mg 1^1) thiamine.HCl 0.4, m-inositol 200, caseine hydrolysate 1000, IAA 0.5, kinetin 10.0; (g 1-1) sucrose 30, bacto agar 6; pH 5.8.
The ’Career’ did not grow on this medium, while the ’Fire flash’ did: 30% of shoots produced an organogenic callus, which was capable of being continuously subcultured. The shoots and plantlets obtained rooted well on a medium containing 0.1 mg 1-1 IAA only, and after a month they were transferred into pots. Callus cultures and in vitro formed plants, examined for Xanthomonas pelargonii and other bacteria, were found to be free of pathogens.
Keywords
Hrčak ID:
158367
URI
Publication date:
31.12.1980.
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