Original scientific paper

Structural and Functional Differentiation of Wheat Chloroplasts

Mercedes Wrischer ; Hrvatska

Abstract

Photooxidation of diaminobenzidine (DAB) has been used to detect ultrastructurally the photosynthetic activity (photosystem I activity) in the thylakoids of differentiating chloroplasts of wheat leaves. It has been shown that the accumulation of photooxidized DAB deposits first in single thylakoids of plastids of cells lying 0.5 to 1 mm above the leaf base. Later the thylakoids multiply and aggregate into grana; in leaf segments being 2 — 3 mm above the leaf base, there are already chloroplasts with an elaborate system of grana and stroma thylakoids, which all show a positive DAB reaction. Plastid envelopes and small prolamellar bodies, which develop in young chloroplasts up to 1 cm from the leaf base, remain without photooxidized DAB deposits. The changes described are discussed in connection with the present knowledge of the functional differentiation of chloroplasts.

Keywords

Hrčak ID:

158428

URI

https://hrcak.srce.hr/158428

Publication date:

31.12.1981.

Article data in other languages: croatian

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