Acta Botanica Croatica, Vol. 45 No. 1, 1986.
Original scientific paper
In vitro Growth and Development of Oaks (Quercus robur and Q. petraea)
Branka Pevalek-Kozlina
; Hrvatska
Sibila Jelaska
; Hrvatska
Abstract
Experiments were carried out to establish an initial culture of Quercus robur and Q. petraea. Apical and axillary buds, taken from seedlings and young plants (up to two years), were used as initial explants for the establishment of culture. The buds were satisfactorily disinfected by successive use of chlorine products and mercuric chloride. Three nutrient media were used: mod. De Fossard medium, ACM medium and mod. WPM medium. Various concentrations of hormones BA, KIN, 2iP, NAA, IBA and GA, were added to all media investigated. Elongation of cultured axillary and apical buds and in some cases adventitious bud induction were achieved when ACM and mod. WPM media were used. Age of explants used did not have an inhibitory effect on positive reaction obtained on both media.
Keywords
Hrčak ID:
159207
URI
Publication date:
31.12.1986.
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