Acta Botanica Croatica, Vol. 45 No. 1, 1986.
Original scientific paper
The Effect of 2,4-D and Kinetin on Soybean Calus Culture
Zdenko Rengel
; Hrvatska
Sibila Jelaska
; Hrvatska
Abstract
Long-term callus cultures of barley (Hordeum vulgare L.) with decreased embryogenic potential were used in testing the influence of L-proline on somatic embryogenesis. The addition of up to 10 mM L-proline to the induction medium increased both the frequency of embryoid formation and the number of subsequently regenerated plantlets. Most of the plantlets (82%), however, were albinos. Some callus lines retained embryoid and plantlet formation capacity after being in culture for more than 21 months.
Keywords
Hrčak ID:
159210
URI
Publication date:
31.12.1986.
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