Original scientific paper

Tumor Transformation in Potato Tuber Tissue

Marijana Krsnik Rasol ; Hrvatska
Tatjana Bakran-Petricioli ; Hrvatska

Abstract

Potato (Solarium tuberosum L. cv. Desirée) tuber tissue was infected with Agrobacterium tumefaciens, strains: B6S3 (wild type), pGV2255 (rooty mutant), pGV2215 (shooty mutant) and with Agrobacterium rhizogenes (8196). Successful tumor induction was achieved by B6S3 (unorganised tumors) and by pGV2255 (rooty tumors). Transformed tuber cells were smaller than normal ones and free from starch. Transformation process caused a significant increase in peroxidase activity which can be taken as a tumorigenetic marker. Electrophoretic protein pattern showed that tumor cells stopped producing a polypeptide of about 40 kd, which was a storage protein dominant in normal tissue.

Keywords

Hrčak ID:

159505

URI

https://hrcak.srce.hr/159505

Publication date:

31.12.1989.

Article data in other languages: croatian

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